trpv1 isg15 p16  (Boster Bio)

Journal: bioRxiv

Article Title: A novel role for TRPV1 in macrophage giant cell formation

doi: 10.64898/2026.05.11.724406

Figure Lengend Snippet: (A) Immunoblot analysis shows TRPV1, TRPA1, and actin levels in WT BMDMs 48 h following treatment with or without IL-4 plus GMCSF (25 ng/ml). (B-C) Densitometric quantification of immunoblot data from (A) (n = 3 biological replicates; one-way ANOVA, **p < 0.01, ***p < 0.001). (D) Representative immunofluorescence images of WT BMDMs stained for TRPV1 (red) using anti-TRPV1 IgG (original magnification, 60 x; scale bar, 2 μm; n = 10 cells per condition). Statistical analysis by Student’s t-test, ***p < 0.001.

Article Snippet: C57BL/6 and TRPV1 KO mice were purchased from The Jackson Laboratory (ME, USA).

Techniques: Western Blot, Immunofluorescence, Staining

Journal: bioRxiv

Article Title: A novel role for TRPV1 in macrophage giant cell formation

doi: 10.64898/2026.05.11.724406

Figure Lengend Snippet: (A) Representative Giemsa-stained images of multinucleated FBGCs in WT BMDMs left untreated or stimulated with IL-4 plus GMCSF (25 ng/ml, 96 h), in the presence or absence of the TRPV1 antagonist AMG. (B-D) Quantitative analysis of FBGC formation from (A): (B) number of FBGCs per high-power field, (C) percentage of fused BMDMs, and (D) average FBGC size. Data represent n = 3 biological replicates with 5 images per group; scale bar, 100 μm; Student’s t-test, ***p < 0.001, ****p < 0.0001. (E) Representative immunofluorescence images of WT BMDMs transfected with scramble or TRPV1-targeting siRNA, stained for TRPV1 (red) using anti-TRPV1 IgG (original magnification, 60x; scale bar, 2 μm). (F) Quantification of TRPV1 fluorescence intensity (n = 10 cells per condition; Student’s t-test, ***p < 0.001). (G) Immunoblot showing TRPV1 expression in WT BMDMs 48 h after transfection with scramble or TRPV1 siRNA. (H-J) Quantification of FBGC formation following TRPV1 knockdown: (H) number of FBGCs per high-power field, (I) percentage of fused BMDMs, and (J) average FBGC size. Data represent n = 3 biological replicates with 5 images per group; Student’s t-test, ***p < 0.001.

Article Snippet: C57BL/6 and TRPV1 KO mice were purchased from The Jackson Laboratory (ME, USA).

Techniques: Staining, Immunofluorescence, Transfection, Fluorescence, Western Blot, Expressing, Knockdown

Journal: bioRxiv

Article Title: A novel role for TRPV1 in macrophage giant cell formation

doi: 10.64898/2026.05.11.724406

Figure Lengend Snippet: (A) Representative images of FBGCs formed by WT BMDMs cultured on collagen-coated (10 μg/ml) PA hydrogels of defined stiffness (1 kPa and 50 kPa), left untreated or stimulated with IL-4 plus GMCSF (25 ng/ml, 96 h), in the presence or absence of the TRPV1 antagonist AMG. (B-C) Quantification of FBGC formation from (A): (B) number of FBGCs per high-power field and (C) percentage of fused BMDMs. (D) Representative images of FBGCs in WT and TRPV1 KO BMDMs cultured on Permanox slides under untreated or IL-4 plus GMCSF-stimulated conditions (25 ng/ml, 96 h). (E-F) Quantification of FBGC formation from (D): (E) number of FBGCs per high-power field and (F) percentage of fused BMDMs. (G) Representative images of FBGCs in WT and TRPV1 KO BMDMs cultured on collagen-coated (10 μg/ml) 50 kPa PA hydrogels, with or without IL-4 plus GMCSF stimulation (25 ng/ml, 96 h). (H-I) Quantification of FBGC formation from (G): (H) number of FBGCs per high-power field and (I) percentage of fused BMDMs. Data represent n = 3 biological replicates with 5 images per group. Scale bar, 20 μm; statistical analysis by one-way ANOVA, ***p < 0.001, ****p < 0.0001.

Article Snippet: C57BL/6 and TRPV1 KO mice were purchased from The Jackson Laboratory (ME, USA).

Techniques: Cell Culture

Journal: bioRxiv

Article Title: A novel role for TRPV1 in macrophage giant cell formation

doi: 10.64898/2026.05.11.724406

Figure Lengend Snippet: (A) Spinning-disk confocal images of WT and TRPV1 KO BMDMs showing Ca 2+ influx (red) under untreated (UT) conditions or following IL-4 plus GMCSF stimulation (25 ng/ml) in response to the TRPV1 agonist capsaicin; scale bar, 50 μm. (B) Quantification of fluorescence intensity from (A) (n = 4 fields per condition). (C) FlexStation 3 measurements of Ca 2+ influx in TRPV1 KO BMDMs stimulated with the TRPV4-specific agonist GSK1016790A (GSK101) under untreated (buffer control) or IL-4 plus GMCSF-treated conditions (25 ng/ml). (D) Quantification of Ca 2+ responses from (C). Experiments were performed three times with quadruplicate measurements. RFU, relative fluorescence units. Statistical significance was determined by Student’s t -test (**p < 0.01, ***p < 0.001).

Article Snippet: C57BL/6 and TRPV1 KO mice were purchased from The Jackson Laboratory (ME, USA).

Techniques: Fluorescence, Control